High-Content Screening

High content screening refers to the simultaneous detection of the effects of screened samples on various aspects of cell morphology, growth, differentiation, migration, apoptosis, metabolic pathways, and signal transduction, while maintaining the integrity of cell structure and function, in order to obtain a large amount of relevant information in a single experiment.Technically speaking,high-content screening is a multiplex and functional screening technique that uses a high-resolution fluorescence digital imaging system to detect multiple indicators at the cellular level, with the aim of obtaining real-time and rapid biological response information of screened samples on cells.

 

Advanced System Configuration

The automated cell imaging system, Operetta CLS, by PerkinElmer in the US, is equipped with four types of lenses – 10X Air, 20X Air, 40X Air, and 63X Water.It offers four imaging modes: bright-field imaging, non-confocal fluorescence imaging, confocal fluorescence imaging, and unlabeled imaging.Furthermore, it also equipped with high-energy solid-state light source covering up to 8 excitation bands,making the dye selection more abundant and flexible, and the high-energy light source ensures a high signal-to-noise ratio of the image.384-well plate testing can be performed to support high-throughput screening efforts.

 

Powerful Analysis Software

The instrument comes equipped with Harmony software, which integrates imaging and analysis into one platform. It features an intuitive user interface, allowing users to develop detection methods, automate hardware processes, analyze data, and store as well as retrieve results seamlessly.

 

List of High-Content Screening Service

1.DNA damage repair

2.Changes in cellular protein expression

3.Cellular protein translocation

4.Cellular protein sub-localization

5.Cellular protein co-localization

6.Cellular cytoskeletal changes

7.Antibody internalization,ect.

8.Identification and analysis of neural cells